Astrocytes synthesize and secrete prostaglandin D synthetase in vitro
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چکیده
منابع مشابه
Human eosinophils synthesize and secrete interleukin-6, in vitro.
Using the technique of in situ hybridization, we have shown that resting, unstimulated, human peripheral blood eosinophils, obtained from subjects with greater than 8% eosinophilia, transcribe and translate messenger RNA (mRNA) for interleukin-6 (IL-6). After incubation for 24 hours in culture medium alone, approximately 19% of eosinophils were positive for IL-6 mRNA. This may be a reflection o...
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Thrombospondin, one of the major glycoproteins released from a-granules of thrombin-stimulated platelets, is a disulfide-linked trimer of 1 60,000-dalton subunits. Cultured human monocytes secreted thrombospondin (determined by an enzyme-linked immunosorbent assay) into the culture medium in a time-dependent manner (1 .45 g/10’ cells/24 hr); secretion was totally blocked by cycloheximide (1 g/m...
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Prostaglandin D synthetase [(5Z, 13E)-(15S)-9 alpha, 11 alpha-epidioxy-15-hydroxyprosta-5,13-dienoate D-isomerase, EC 5.3.99.2] activity was found in the high-speed (100,000 x g, 1 hr) supernatant of the homogenate of the cochlea of adult rats. The specific activity (14.0 nmol/min per mg of protein) was 6- to 7-fold higher than that in the central nervous system. The enzyme showed full activity...
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research
سال: 1996
ISSN: 0167-4889
DOI: 10.1016/0167-4889(95)00182-4